<p>Nitrophorins are haemoproteins found in saliva of blood-feeding insects [<cite idref="PUB00008042"/>, <cite idref="PUB00008043"/>]. Saliva of the blood-sucking bug <taxon tax_id="13249">Rhodnius prolixus</taxon> (Triatomid bug) contains four homologous nitrophorins, designated NP1 to NP4 in order of their relative abundance in the glands [<cite idref="PUB00008044"/>]. As isolated, nitrophorins contain nitric oxide (NO) ligated to the ferric (FeIII) haem iron. Histamine, which is releasedby the host in response to tissue damage, is another nitrophorin ligand. Nitrophorins transport NO to the feeding site.Dilution, binding of histamine and increase in pH (from pH ~5 in salivary gland to pH ~7.4 in the host tissue) facilitate the release of NO into the tissue where it induces vasodilatation.</p><p>The salivary nitrophorin from the hemipteran <taxon tax_id="79782">Cimex lectularius</taxon> (Bed bug) has no sequence similarity to R. prolixus nitrophorins. It is suggested that the two classes of insect nitrophorins have arisen as a product of the convergentevolution [<cite idref="PUB00008045"/>].</p><p>3-D structures of several nitrophorin complexes are known [<cite idref="PUB00008043"/>]. The nitrophorin structures reveal lipocalin-likeeight-stranded beta-barrel, three alpha-helices and two disulphide bonds, with haem inserted into one end of the barrel. Members of the lipocalin family are known to bind a variety of small hydrophobic ligands, including biliverdin, in a similar fashion (see [<cite idref="PUB00000545"/>] for review). The haem iron is ligated to His59. The position of His59 is restrained through water-mediatedhydrogen bond to the carboxylate of Asp70. The His59-Fe bond is bent ~15 degrees out of the imidazole plane. Asp70 forms an unusual hydrogen bond with one of the haem propionates, suggesting the residue has an altered pKa. In NP1-histaminestructure, the planes of His59 and histamine imidazole rings lie in an arrangement almost identical to that found in oxidised cytochrome b5.</p>This entry represents the nitrophorin family. Nitrophorin